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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
hypodermic needle Autoclip metal wound clipper 1 serafin clip 1 mouth pipetter and hand-pulled transfer pipette The Anesthetic Avertin (2,2,2 tribromoethanol) is found to be quite effective. For method of preparation, see "Manipulating the Mouse
machine. SKI STAINING MACHINE 1. Remove the new slides from box. 2. Clip them individually onto the ski using the correct clips x25 slides maximum. 3. The procedure takes approximately 20 minutes, and the slides pass
Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy
. Here, we describe the purification of EB1 and CLIP-170, the total internal reflection fluorescence microscopy assay to observe dynamic end tracking in vitro, and the quantitative analysis of fluorescent +TIP comet shape and of single +TIP molecule turnover at growing microtubule
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