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文献和实验ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
4 2 x 106 EP + DNA Normal % survival after EP + DNA 5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
5 2 x 106 EP + DNA G418 % total integrants ie random + H/R's 6 2 x 106 EP + DNA G418* Same as Plate 5 unless 7 2 x 106
A safe method of extracting DNA from Coccidioides immitis
Human-pathogenic fungi such as Coccidioides immitis and Histoplasma capsulatum must be handled in Biosafety level 3 containment facilities which make for a very awkward working environment (J.Y. Richmond and R.W. McKinney, eds. Biosafety
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