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- merck-millipore
- CS210094
- 2025年09月26日
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文献和实验7 or SP6 Kit from PROMEGA Glutathion-Agarose beads from Pharmacia Protocol: a) Resin preparation (50% slurry): 1- Transfer a 1.33 mL aliquot of the resin (mix well before use) into a 15 mL Falcon tube 2- Centrifuge (1pulse at 500g) 3- Resuspend in 10
Coupling Peptides To Resin For Affinity Purification
is light sensitive. Wash with 10 volumes of 0.1 M NaPi pH 7.8= buffer. Resuspend the resin as a 50% slurry in buffer. Add the peptide. If the peptide is soluble directly in buffer, add it as a solid. Many peptides go in better added as a 100
Purification of GST fusion proteins in E.coli GST融合蛋白纯化（方法四）筛选表达
aliquots of the insoluble material for analysis by SDS-PAGE.Transfer the supernatants to fresh tubes,Add 20 μl of a 50% slurry of Glutathione Sepharose 4B (prepared as described above)to each supernatant and mix gently for 5 min at r.t..Add 100 μl of 1 x
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