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文献和实验Characterization of GABA Receptors
for protein assay ( appendix 3A ) Alternate Protocol 1: Measurement of GABAA Receptor Binding in Rat Brain Membranes Using [3H]GABA
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
Step 3: 72.0ºC for 00:30 Cycle 3: ( 80X) Step 1: 55.0ºC for 00:10 Increase setpoint temperature after cycle 2 by 0.5ºC Melt curve data collection and analysis enabled
[分享] Strip Antibodies and Re-use Protein blots membrane
/5% H2O2 3) rinse in H2O for few times 4) 5 min in PBS, then ready. Two: 1. Make Strip solution: 2% SDS 100mM beta-mercaptoethanol, or DTT 20mM Tris-Cl 1mM EDTA 2. Rinse the membrane with 100% methonal for few min. 3. Rinse 5X in H2O 4. Wash
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