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- merck-millipore
- MABE363
- 2025年09月02日
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文献和实验Clone information and RNAi Feeding Protocol
AHRINGER LAB Clone information and RNAi Feeding Protocol Vector and inserts: Genomic fragments obtained by PCR were cloned into the Timmons and Fire feeding vector (L4440
Reverse Sequencing of M13 Cloned DNA
) or there may be gaps in the sequence. In both of these circumstances, it can be of benefit to be able to determine the sequence from the opposite strand of an Ml3 clone, or to be able to get sequence information from the opposite end of a large insert (1 ,2 ).
・ M13 Phage (Michael Blaber) Very useful background information about M13: its infection, replication, packing, cloning. If you are new to phage culture, it will be great beneficial to read this. ・ Large Scale M13
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