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文献和实验Transfection of E. coli with M13 DNA
In recent years several techniques have been described for the introduction of DNA molecules into strains of E. coli by transformation or transfection. These are based on the findings of Mandel and Higa (1 ) who demonstrated that incubation
Transformation of E. coli by Electroporation
Materials: SOB medium E. coli host strain such as DH5α WB tRNA 5 M ammonium acetate
electroporate @ 2.5kV,25 uF,200 ohms (expect time constant 3.5-5msec) 11.IMMEDIATELY add 1ml cold SOC.Transfer to eppendorf. 12.37℃/60' 13.Plate 50-200μl cells (50 ~ 200-300 colonies) Dr.DE Koshland,Carnegie Institution of Washington http://www.ciwemb.edu
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