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- merck-millipore
- MAB2260
- 2025年08月27日
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文献和实验Cell Surface Marker and Cell Cycle Analysis, Hedgehog Signaling, and Flow Cytometry
in the development of some neoplasias. Hh signaling affects the expression of cell surface markers of differentiation.We have used the flow cytometer to evaluate the effect of blockage of the Hh signal on the expression of cell surface markers of erythroid
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Cell Surface Immunofluorescence Staining Protocol
as the antibodies used for immunofluorescent staining. 4. Cell-Surface Staining with Antibody: Add appropriately conjugated fluorescent, biotinylated, or purified primary antibodies at predetermined optimum concentrations (e.g. anti-CD3-FITC, anti
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