Complement, Rabbit Serum

Measurement of Complement Hemolytic Activity, Generation of Complement-Depleted Sera, and Production of Hemolytic Intermediates

All of the basic functional assays of complement activity utilize erythrocytes as targets (1 ). For classical pathway assays, the favored target is the antibody-sensitized sheep erythrocyte. For alternative pathway assays, unsensitized rabbit

Complement and Immune Complexes

Serum sickness resulting from repeated administration of horse antitoxins was hypothesized to be an immune complex disease by von Pirquet and Schick at the turn of this century (1 ). Arthus developed the first animal model of immune complex

Complement-Dependent Cytotoxicity Crossmatch

are present in patient serum, these antibodies can bind donor cells. Antibody-antigen complexes, in turn, can activate the complement cascade, leading to complement-mediated cytotoxicity. Two commonly performed cytotoxic crossmatches, using donor lymphocytes