- 询价
- merck-millipore
- 90499-K
- 2025年07月10日
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北京盛科博源生物科技有限公司
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文献和实验Alkaline Southern Blotting Procedure
and an additional 1 sec on gel alone. Invert gel, place into .25M HCl for 7 min. Rinse briefly in dH2O. Soak in 0.3M NaOH,0.3M NaCl for 10 min. Set up a transfer tray (9 X 12 inch glass baking dish) by pouring transfer solution in the tray (about 1000 ml
Steady State ATPase Assays Coupled Enzyme System
from Rabbit Muscle in 50% glycerol) Procedure 1. Assemble microtubules for assays. E.g., 58 µL of 50 µM MTs = 50 µL 5.8 mg/mL tubulin + 0.5 µL 100 mM Mg・GTP, incubate for 30-60 min at 37°C
Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
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