- 询价
- merck-millipore
- PMEF-N-K
- 2025年07月11日
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文献和实验of some supernatant. In order to lyse Staphylococc us, proteinase K was added to the 5% Chelex 100 buffer, and the mixture was incu bated at 56℃ for 60 min and then boiled for 15 min. Blood samples in the prese nce of heparin, were subjected to red cell lysis
the flask for 38 minutes. (3000 rads required. 1 gray=1.2718 min, 1 gray=100 rads). Once irradiated, wipe flask with 70% ethanol and return to incubator until ready to trypsinise. TRYPSINISING CELLS Aspirate the PMEF media from the flask
the cell suspensions in a falcon tube and irradiate that. - Irradiate the flask for 41 minutes. (3000 rads required. 1 gray=1.376 min (Oct. 98), 1 gray=100 rads). - Once irradiated, wipe flask with 70
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