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- 2025年07月14日
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文献和实验Construction of Retro viral Packaging Cell Lines
Most of the time, retrovirus vectors retain only cis -acting sequences from the original viral genome. These sequences allow the recombinant structure to be transcribed (LTR promoter/enhancer) and the RNA to be processed (splicing
Guidelines for Retro-orbital Bleeding in Laboratory Rats and Mice
and experienced individual(s) to perform this technique without anesthesia, following observation and assessment of skill by an IACUC designee. 3. Due to the nature and anatomy of rat tissues, retro-orbital
Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Successful retroviral gene transfer into hematopoietic cells has been demonstrated in a number of small and large animal models and clinical trials. However, severe adverse events related to insertional muta-genesis in a recent clinical trial
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