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文献和实验Coupling Peptides To Resin For Affinity Purification
volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add
Coupling Peptides To Resin For Affinity Purification
Coupling Peptides To Resin For Affinity Purification Claire Walczak January 1996 We use Affigel-10, converting its functional group first to amino and then to iodoacetyl. You can also buy amino resin (it's
the protein sample.MALDI targetMicrocentrifugeMicrocentrifuge tubes (standard and 0.7-mL)METHODFigure 1. Schematic of affinity binding of phosphopeptides to immobilized metal ion affinity columns.Preparation of the Immobilized Metal Ion Affinity Column Insert
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