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- 详细信息
- 文献和实验
- 技术资料
- 服务名称:
Tumor Suppressor Genes DNA Methylation PCR Array
- 提供商:
SAB
Cell Adhesion: APC, CDH1, CDH13, CDKN2A.
Cell Cycle: APC, BRCA1, CDKN2A, FHIT, MLH1, NEUROG1, PTEN, RASSF1, RUNX3, TP73, VHL.
DNA Damage & Repair: APC, BRCA1, MGMT (AGT), MLH1, TP73.
Signal Transduction: APC, BRCA1, CDH13, ESR1, PTEN, RASSF1, SOCS1, WIF1.
Transcription Factors: BRCA1, CDH1, ESR1, NEUROG1, RARB, RUNX3, TP73, VHL.
Other: PDLIM4 (RIL).
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文献和实验Identification of DNA-Binding of Tumor Suppressor Genes by Chromatin Immunoprecipitation
fragment with the protein upon antibody binding. Here we describe a protocol for the immunoprecipitation of transcription factors bound to promoter sequences in cells, and use the p53 tumor suppressor as an example (Fig. 1 ). Fig. 1.
, and hypermethylation of the promoter regions is associated with gene silencing. Hypermethylation of tumor suppressor genes and other CpG island genes, such as p15, p16 , estrogen receptor, E-cadherin, VHL, HIC-1 , and retinoblastoma and corresponding transcriptional
Colony Growth Suppression by Tumor Suppressor Genes
to analyze cell cycle arrest and apoptosis induction to characterize the growth inhibition activity of tumor suppressor genes, including flow cytometry assay (6 ), MTT assay (7 ), and clonogenic survival assay (8 ). Among these, the clonogenic survival
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