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- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
6 months from date of receipt.
- 英文名:
Mouse IgG protein
- 库存:
99
- 供应商:
Biorbyt
- 规格:
10 mg
描述: Chromatographically purified Mouse IgG. Purified Mouse IgG may be used as a reference antigen, calibrator, coating protein and blocking agent in a variety of immunoassays including immunodiffusion, immunoelec- trophoresis, ELISA, Western blotting, dot-immunobinding assays (DIBA), haemagglutination, and cell-binding assays.
形态: Lyophilized, stable white powder.
RRID: AB_10748660
特异性: Chromatographically purified Mouse IgG
研究领域: Infectious Disease & Virology
保存条件: This product is shipped lyophilized at ambient temperature. After arrival store at +4°C. Prolonged storage may be at -20°C. Allow to stand at ambient temperature for 5-10 minutes to reach equilibrium. Reconstituted solution may be stored frozen. Working dilutions may be prepared by adding the required amount of phosphate buffered saline (PBS), should not be refrozen and preferably used within 24 hours. Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product.
备注: For research use only.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
Protein Therapeutics: Mouse, Humanized, and Human Antibodies
Antibodies play a vital role in immune defense against pathogens. They are globular glycoproteins produced by plasma cells in response to the antigenic stimulation of B-lymphocytes. Their circulation in blood and lymph contributes to the humoral
Surface plasmon resonance (SPR) is a key technology to evaluate IgG effector functions in vitro involving binding to Fcgamma receptors (FcγR). We describe here the chemical coupling of protein A to a sensor chip. IgGs prepared
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