- ￥5031
- Biorbyt
- orb154501
- 英国
- 2026年07月22日
- FC
- Feline, Ferret, Human, Primate, Rabbit
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- 文献和实验
- 技术资料
- 抗体名：
CD271 Antibody (PE) 抗体
- 抗体英文名：
CD271 Antibody (PE)
- 靶点：
CD271
- 应用范围：
FC
- 适应物种：
Feline, Ferret, Human, Primate, Rabbit
- 保质期：
12 months from date of receipt.
- 抗原来源：
详询
- 目录编号：
orb154501
- 级别：
科研
- 库存：
99
- 供应商：
Biorbyt
- 标记物：
PE
- 克隆性：
Monoclonal
- 保存条件：
详询
- 亚型：
Mouse IgG1
- 免疫原：
详询
- 规格：
100 T
产品子类型: Primary Antibody
描述: Mouse monoclonal antibody conjugated to PE which recognizes CD271 that is low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. It can mediate cell survival as well as cell death of neural cells. CD271 is useful for the detection of all cells expressing p75NTR on their surface, such as motor neurons cholinergic basal forebrain neurons, activated Schwann cells, olfactory ensheathing glial cells.
克隆号: NGFR5
别名: TNFRSF16, NGFR, p75NTR, p75NGFR
应用说明: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.
免疫原: Purified CD271 protein isolated from human melanoma cell line A875
防腐剂: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
纯化方法: Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
特异性: The mouse monoclonal antibody NGFR5 (originally C34C) recognizes an epitope within ammino acids 1 - 160 of CD271/NGFR, a 75 kDa transmembrane glycoprotein of the TNFR superfamily.
研究领域: Immunology & Inflammation; Cell Biology
UniProt ID: P08138
Entrez 基因 ID: 4804
保存条件: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
备注: For research use only.
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文献和实验Western Blotting with Horseradish Pe
7.5, 100mM NaCl, 0.1% Tween 20). 2.Incubate the blot for 30 minutes at 37℃, 1 hour at room temperature, or overnight at 4℃. INCUBATION WITH HORSERADISH PEROXIDASE CONJUGATED ANTIBODY NOTE: THE INCLUSION OF SODIUM AZIDE IS TO BE AVOIDED IN ALL STEPS
Purification of Immature CD4+CD8+ Thymocytes by Panning with Anti-CD8 Antibody
Most T lymphocytes of the immune system differentiate within the thymus along the CD4/CD8 developmental pathway by a highly ordered process termed thymic selection ( 1 , 2 ). The maturation status of thymocytes is commonly assessed
流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学，对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型，是流式中最常见的实验之一。 通常，我们会先检测 CD3，来圈总T淋巴细胞，再分别用 CD4 抗体和 CD8 抗体，来区分 CD4+ T 淋巴细胞（辅助或调节性T细胞）和 CD8+ T 淋巴细胞（细胞毒性 T 细胞）。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出，在新生小鼠脾脏中，绝大
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