- 询价
- Liver miFinder miRNA PCR Array(肝脏发现者microRNA芯片)
- 上海
- 2025年12月31日
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- 详细信息
- 文献和实验
- 技术资料
- 服务名称：
Liver miFinder miRNA PCR Array(肝脏发现者microRNA芯片)
- 提供商：
SAB
| Human Liver miFinder miRNA PCR Array: MIHS-116Z | |||||||||||||||||
The Human Liver miFinder miScript miRNA PCR Array profiles the expression of 84 miRNAs abundantly expressed or best characterized in liver tissue. As the number of annotated miRNAs continues to expand, it becomes increasingly important to focus only on those miRNAs expressed in a particular cell line or tissue of interest, as not all miRNAs are expressed in every tissue. Due to this, we have experimentally analyzed the expression of miRNAs identified through miRBase V18 in a pool of human liver samples and correlated the results with scientific literature. Each of these miRNAs can regulate one or more messenger RNA transcripts, and conversely a given mRNA can be regulated by one or more miRNAs. Therefore, although they are well characterized, the complex role played by each miRNA is yet to be completely defined. This array maximizes the likelihood of discovering miRNAs whose expression patterns correlate with the biological phenotypes of liver tissue or hepatic cell lines under study. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs expressed in liver tissue can be easily and reliably analyzed with this miScript miRNA PCR Array.
96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available. |
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文献和实验和mRNA 表达差异，希望能找到耐受性差异的分子机制。首先作者利用Agilent Human miRNA Microarray V2.0 分析这6 种人类胃癌细胞microRNA 的表达情况，找到了25 个microRNA，这些microRNA 的表达丰度与HCPT耐受性的强弱有关。然后利用Affymetrix HG‐U133+ PM Array Plate 分析mRNA 的表达情况，结果找到了307 个与HCPT 耐受能力相关的mRNA，其中33 个基因曾经被报道与肿瘤形成、细胞增殖有关。
提供的PCR Array产品举例 订制的PCR芯片 如果现有的产品无法满足研究者的特定需要，SuperArray还可以提供从设计到芯片生产的完整服务，为研究者提供使用先进的PCR芯片的便捷服务。客户订制芯片服务为研究者提供以下便利：1）在使用表达谱基因组芯片后，对从基因组水平筛选出来的一组基因进行验证；2）在现有产品的基础上作适当调整以适应特殊需要；3）完全从头设计，适用于在现有的预设计PCR芯片中尚未包括的某个信号通路或者一组基因。若研究基因数目少于84个，还可以将96孔PCR芯片进一步分成
分析，要不你就要在你做的里面挑几个做后续的功能研究包括靶标的寻找。或者你看看你做的microRNA之间是否有什么联系存在，这个比较异想天开了，毕竟microRNA之间不会直接相互作用了。 ebio66 上海生物芯片提供aglient的miRNA芯片服务，结果准确可靠。 clion007 如果你的钱多，就最好用PCR咯！ 比起芯片来，我们的qPCR灵敏度和特异性都好很多。 芯片的假阳性会比较高，特异性是很差
