纤维化miRNA PCR芯片 Fibrosis miRNA

The Human Fibrosis miScript miRNA PCR Array profiles the expression of 84 miRNAs known to play a role in fibrosis. During tissue remodeling that occurs as part of wound healing, apoptosis removes myofibroblasts, and the deposited extracellular matrix (ECM) is remodeled to resemble the original tissue. Fibrosis occurs when inappropriate tissue remodeling results in excess ECM deposition due to inappropriate survival of myofibroblasts or lack of ECM proteolytic degradation. The inflammation and enhanced TGFβ signaling often present in fibrotic tissues causes cells to differentiate into myofibroblasts via epithelial-to-mesenchymal transition (EMT). miRNAs may play a role in conducting the pathogenesis of fibrosis by modulating the expression of genes involved in ECM remodeling and cell adhesion, inflammation, angiogenesis, TGFβ/CTGF signaling, and EMT. This array includes miRNAs that are differentially expressed during fibrosis and are known or predicted to modulate the expression of pro- and anti-fibrotic genes common to different fibrotic disorders such as systemic sclerosis, as well as liver, cardiac, kidney, and idiopathic pulmonary fibrosis. The profiling results from this array may yield insights into the molecular mechanisms behind the pathogenesis of fibrosis. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR Green real-time PCR, the expression of a focused panel of miRNAs involved in fibrosis can be easily and reliably analyzed with this miScript miRNA PCR Array. miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease. 96-well, 384-well ( 4 x 96 ), and Rotor-Disc 100 formats are available.

Pro-Fibrotic: miR-142-3p , miR-145-5p, miR-155-5p, miR-199b-5p, miR-21-5p, miR-215, miR-216a-5p, miR-27a-3p, miR-27b-3p, miR-32-5p, miR-338-5p, miR-34a-5p, miR-377-3p, miR-382-5p, miR-192-5p, miR-30a-5p, miR-208a, miR-5692a, miR-5011-5p.
Anti-Fibrotic: let-7d-5p, miR-107, miR-132-3p, miR-133a, miR-141-3p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-18a-5p, miR-194-5p, miR-19a-3p, miR-19b-3p, miR-200a-3p, miR-200b-3p, miR-204-5p, miR-20a-5p, miR-211-5p, miR-26a-5p, miR-26b-5p, miR-29b-3p, miR-335-5p, miR-449a, miR-449b-5p, miR-590-5p, miR-92a-3p.
Extracellular Matrix Remodeling & Cell Adhesion: miR-199a-5p, miR-199b-5p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-449a, miR-21-5p, miR-204-5p, miR-25-3p, miR-7-5p, miR-196a-5p, miR-203a, miR-27b-3p, miR-27a-3p, miR-451a, miR-145-5p, miR-143-3p, miR-1, miR-10a-5p, miR-10b-5p, miR-661, miR-30a-5p, miR-16-5p, miR-217.
Inflammation: let-7d-5p, miR-204-5p, miR-122-5p, miR-146a-5p, miR-199a-5p, miR-155-5p, miR-21-5p, miR-129-5p, miR-142-3p, miR-503-5p.
Angiogenesis: let-7d-5p, miR-378a-3p, miR-372, miR-34a-5p, miR-29b-3p, miR-20a-5p, miR-200b-3p, miR-195-5p, miR-17-5p, miR-16-5p, miR-15b-5p, miR-150-5p, miR-145-5p, miR-126-3p, miR-107, miR-31-5p, miR-375.
Signal Transduction & Transcriptional Regulation: miR-26a-5p, miR-18a-5p, miR-122-5p, miR-133a, miR-20a-5p, miR-203a, miR-19a-3p, miR-17-5p, miR-146a-5p, miR-155-5p, miR-92a-3p, miR-29b-3p, miR-141-3p, miR-29a-3p, miR-204-5p, miR-590-5p, miR-372, miR-302b-3p, miR-19b-3p, miR-21-5p, miR-1, miR-217, miR-23a-3p, miR-328, miR-744-5p, miR-148a-3p, miR-451a, miR-125b-5p, miR-449a, miR-34a-5p, miR-29c-3p, miR-195-5p, miR-192-5p, miR-16-5p, miR-15b-5p, miR-663a, miR-30a-5p, miR-378a-3p, miR-145-5p, miR-146b-5p, miR-27a-3p, miR-324-5p, miR-324-3p, miR-223-3p, miR-491, miR-101-3p, miR-491-5p.
Epithelial-to-Mesenchymal Transition: let-7d-5p, miR-107, miR-155-5p, miR-199a-5p, miR-199b-5p, miR-200a-3p, miR-200b-3p, miR-215, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-382-5p, miR-325, miR-874.