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NR2B Antibody抗体,orb67425,biorb

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  • ¥7670
  • biorbyt已认证
  • orb67425
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, IP, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体名

      NR2B Antibody抗体

    • 抗体英文名

      NR2B Antibody

    • 靶点

      NR2B

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, IP, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb67425

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Monoclonal

    • 亚型

      IgG2b

    • 免疫原

      Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B

    • 规格

      100 ug

    产品描述:Mouse Anti-Rat GluN2B Monoclonal IgG2b

    别名:NMDAR2B, hNR3, MGC142180, glutamate receptor subunit epsilon-2, NR3

    免疫原:Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B

    克隆性:S59

    分子量:166kDa

    应用稀释比例:WB (1:1000), IHC-P (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-337 was sufficient for detection of NR2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated

    纯化:Protein G Purified

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    NCBI:NP_036706.1

    Entrez:24410

    UniProt ID:Q00960

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NR2B Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.

    产品细节图片3
    Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.

    产品细节图片4
    Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

    产品细节图片5
    Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.

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    • 作者
    • 内容
    • 询问日期
    图标文献和实验
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    • Pharmacological Analysis of Recombinant NR1a/2A and NR1a/2B NMDA Receptors Using the Whole-Cell Patch-Clamp Method

      favorable side-effect profile. We applied the whole-cell patch-clamp technique for testing NR2B subunit-specific drugs in HEK cells transiently or stably expressing different types of NMDAR subunits. In stable cell lines, we applied an inducible mammalian

    • Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII)

      to the NMDA-receptor subunit GluN2B (formerly known as NR2B). Here we discuss studying CaMKII by inhibition (including by the novel class of CN inhibitors) and by mutagenesis. We describe purification of CaMKII, activity assays, directing and probing auto

    • 热点关注:2005年学习和记忆研究进展

      对于神经生物学来讲,研究者们最关心的还是学习记忆相关的蛋白质以何种方式参与了学习记忆,这也将是后基因组时代的一个主流研究方向。复旦大学李葆明的研究小组发现大脑ACC区的NR2B蛋白和恐惧记忆相关,文章发在2005年的《神经元》上。早些年,美国普林斯顿大学的钱卓教授就通过NR2B的过表达创造出了世界上第一个聪明鼠模型,但NR2B在不同脑区的不同功能还是不清楚,ACC是目前最吸引人的一个脑区,美国科学家认为它可能和第六感相关。这篇文章是复旦大学和韩国及加拿大的研究机构合作的,为中国科学研究的高水平

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