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NR2B Antibody (HRP)抗体,orb14875

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  • ¥8320
  • biorbyt已认证
  • orb148758
  • 英国
  • 2025年12月04日
  • AM, ICC, IF, IHC, IP, WB
  • Mouse
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      NR2B Antibody (HRP)抗体

    • 抗体英文名

      NR2B Antibody (HRP)

    • 靶点

      NR2B

    • 浓度

      1 mg/ml

    • 应用范围

      AM, ICC, IF, IHC, IP, WB

    • 宿主

      Mouse

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb148758

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      HRP

    • 克隆性

      Monoclonal

    • 亚型

      IgG2b

    • 免疫原

      Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B

    • 规格

      100 ug

    产品描述:Mouse monoclonal antibody against GRIN2B conjugated to HRP

    别名:NMDAR2B, hNR3, MGC142180, glutamate receptor subunit epsilon-2, NR3

    免疫原:Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B

    克隆性:S59

    分子量:166kDa

    应用稀释比例:WB (1:1000), IHC (1:1000), ICC/IF (1:100)

    应用注释:1 µg/ml of SMC-337 was sufficient for detection of NR2B in 10 µg of rat brain lysate by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

    防腐剂:73.64mM Carbonate, 54.55mM Ethanolamine, 45.45mM Cyanoborohydride, 18.18mM Sodium Hydroxide 0.23mM Citrate

    纯化:Protein G Purified

    保存说明:Conjugated antibodies should be stored according to the product label

    NCBI:NP_036706.1

    Entrez:24410

    UniProt ID:Q00960

    Note:For research use only.

    产品细节图片1
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) GluN2B/NR2B Antibody (D) Composite.

    产品细节图片2
    Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Everything positive.

    产品细节图片3
    Western Blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:250.

    产品细节图片4
    Western Blot analysis of Rat brain membrane lysate showing detection of GluN2B/NR2B protein using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Load: 15 μg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

    产品细节图片5
    Immunohistochemistry analysis using Mouse Anti-GluN2B/NR2B Monoclonal Antibody, Clone S59-36. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-GluN2B/NR2B Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Filaggrin-like staining, and dermal staining.

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    图标文献和实验
    相关实验
    • Conjugation of Antibody to HRP

      of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator.  Desalt the HRP on PBS equilibrated column.  Collect

    • Conjugation of Antibody to HRP

        Conjugation of Antibody to HRP Author: Nanci Donacki Source: Contributed by Nanci Donacki     Reagents

    • Monoclonal Antibody Production Protocol

      - PBS + 0.1% Tween 20 or TBS + 0.1% Tween 20. - Blocking solution = 2% BSA (type V ) in PBS. (Add 0.02% azide for longer storage.) - Elisa buffer = 2% BSA + 0.1% Tween 20 in PBS ( azide optional ). - Enzyme linked antibody = Horseradish peroxidase

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