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Nucleolin/C23 Rabbit Polyclona

l Antibody抗体,orb2698,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb2698
  • 英国
  • 2025年12月31日
  • FC, IF, IHC-Fr, IHC-P
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Nucleolin/C23 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Nucleolin/C23 Rabbit Polyclonal Antibody

    • 靶点

      NCL

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb2698

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human Nucleolin (601-710/710aa)

    • 规格

      50 ul

    产品描述:Nucleolin/C23 Rabbit Polyclonal Antibody

    别名:C23; NCL; Nucl; NUCL_HUMAN; Nucleolin; Protein C23.

    免疫原:KLH conjugated synthetic peptide derived from human Nucleolin (601-710/710aa)

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine

    分子量:76 kDa

    应用稀释比例:IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    UniProt ID:P19338

    Note:For research use only.

    产品细节图片1
    Blank control (blue): Hela (fixed with 2% Fixative (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Phospho-c-Fos (Ser32) /AF488 antibody, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/AF488 (orange), used under the same conditions.

    产品细节图片2
    Blank control: Molt4. Primary Antibody (green line): Rabbit Anti-Nucleolin/C23/FITC Conjugated antibody, dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-FITC. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. The cells were stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片3
    Tissue/Cell: rat uterus tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Nucleolin/C23 Polyclonal Antibody, Unconjugated (orb2698) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
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