- ￥3120
- biorbyt
- orb1266
- 英国
- 2025年12月03日
- ELISA, IF, IHC-Fr, IHC-P, WB
- Rabbit
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Osteocalcin Rabbit Polyclonal Antibody抗体
- 抗体英文名：
Osteocalcin Rabbit Polyclonal Antibody
- 靶点：
BGLAP
- 浓度：
1mg/ml
- 应用范围：
ELISA, IF, IHC-Fr, IHC-P, WB
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1266
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 形态：
Liquid
- 亚型：
IgG
- 免疫原：
KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa)
- 规格：
50 ul
产品描述：Osteocalcin Rabbit Polyclonal Antibody
别名:OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;
免疫原：KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa)
预测反应性:Rat
分子量:11 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:39476113, 40583147
UniProt ID:P02818
Note:For research use only.
别名:OSTCN_HUMAN; BGLAP; Bone Gla protein; BGP; Gamma-carboxyglutamic acid-containing protein; bone gamma-carboxyglutamate protein; OC; OCN;
免疫原：KLH conjugated synthetic peptide derived from human Osteocalcin (21-100/100aa)
预测反应性:Rat
分子量:11 kDa
应用稀释比例：WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
纯化:Affinity purified by Protein A
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:39476113, 40583147
UniProt ID:P02818
Note:For research use only.
citrate buffer (pH6) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nu paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium clei.
Fixative-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.
Fixative-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
Fixative-fixed, paraffin embedded (human skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:100 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei stainin.
Fixative-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Osteocalcin) Polyclonal Antibody, Unconjugated (orb1266) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Sample: Lane 1: Mouse NIH/3T3 cell lysates, Lane 2: Human MG63 cell lysates, Lane 3: Human A549 cell lysates, Lane 4: Human U-2 OS cell lysates, Primary: Anti-Osteocalcin (orb1266) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 11 kDa, Observed band size: 11 kDa.
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文献和实验该产品被引用文献
Fábio Vieira de Miranda-Filho 1, Stéfany Barbosa 1, Olavo Alcalde Panigali 1, Mirela Caroline Silva 1, Monique Gonçalves da Costa 1, Franciele da Silva Flores 1, Edilson Ervolino 2, Letícia Helena Theodoro 1, Osvaldo Magro-Filho 1, Leonardo Perez Faverani 1 Effect of local and systemic administration of atorvastatin for improving bone healing on critical defects Braz Dent J ., (2024), Kai Zheng 1 2, Wenming Li 1, Tianhao Wang 3, Gaoran Ge 1, Wei Zhang 1, Yi Qin 1, Wenhao Li 1, Zebin Wu 1, Zhen Wang 4, Gang Rui 2, Yaozeng Xu 1, Dechun Geng Dopamine D1 Receptor Contributes to Glucocorticoid-Associated Osteonecrosis of Femoral Head Protection Through the ATF3/CHOP Axis to Inhibit Osteoblastic Apoptosis Adv Sci (Weinh), (2025)
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Osteocalcin Rabbit Polyclonal Antibody抗体，orb1266，biorbyt
￥3120
