- ￥6500
- biorbyt
- orb1184754
- 英国
- 2025年12月07日
- ICC, IF, IHC, WB
- Mouse
- Human, Mouse, Rat
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- 详细信息
- 技术资料
- 抗体名：
AREB6/ZEB1 Antibody (monoclonal, 8B12D7)抗体
- 抗体英文名：
AREB6/ZEB1 Antibody (monoclonal, 8B12D7)
- 浓度：
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
- 应用范围：
ICC, IF, IHC, WB
- 宿主：
Mouse
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb1184754
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Monoclonal
- 形态：
Lyophilized
- 亚型：
Mouse IgG2b
- 免疫原：
A synthetic peptide corresponding to a sequence in the middle region of human AREB6/ZEB1.
- 规格：
100 ug
产品描述：Anti-AREB6/ZEB1 Antibody (monoclonal, 8B12D7). Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
别名:Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDH2; ALDM
免疫原：A synthetic peptide corresponding to a sequence in the middle region of human AREB6/ZEB1.
交叉反应性:No cross-reactivity with other proteins.
克隆性:8B12D7
分子量:200 kDa
应用注释：Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
纯化:Immunogen affinity purified.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P37275
Note:For research use only.
别名:Aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDH2; ALDM
免疫原：A synthetic peptide corresponding to a sequence in the middle region of human AREB6/ZEB1.
交叉反应性:No cross-reactivity with other proteins.
克隆性:8B12D7
分子量:200 kDa
应用注释：Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Immunofluorescence, 5 μg/ml, Human. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
纯化:Immunogen affinity purified.
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
UniProt ID:P37275
Note:For research use only.
IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 µg/mL mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. DyLight®550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IF analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 µg/mL mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Biotin conjugated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®550 Conjugated Avidin. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human glioblastoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
IHC analysis of AREB6/ZEB1 using anti-AREB6/ZEB1 antibody. AREB6/ZEB1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml mouse anti-AREB6/ZEB1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.
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AREB6/ZEB1 Antibody (monoclonal, 8B12D7)抗体，orb1184754，biorbyt
￥6500
