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- 2026年01月07日
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- 技术资料
- 库存：
大量
- 供应商：
西宝
- 英文名：
NucleoBond Buffer S1
- 规格：
500 ml
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文献和实验digested plasmid (cut 5 µg of plasmid in volume of 20 µl) 51 µl labeled oligo 10 µl 10X Taq Buffer 6 µl 25 mM MgCl2 10 µl DMSO 2 µl 10 mM dNTP's 1 µl Taq polymerase 14 cycles of PCR Run on 6-8% polyacrylamide/urea gel Cut out probe and elute S1
Nucleobond Column BAC DNA Purification for Transgenic Mouse Production
from Clontech. NucleoBond AX 500 Tip, Clontech Cat. # 4003-1 This gives the best compromise of volume management and size (capacity). This kit contains 10 AX-500 cartridges. NucleoBond Buffer Set I , Clontech Cat. # 4040-1. Without the Buffer Set
1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract and NaOAc/EtOH ppt. 2. Prepare one S1 nuclease tube (on ice) for each time point: 15ml S1 Buffer + 0.25U S1/ml (5U). 3. Use 5ml (=0.5mg) digested plasmid (in EB) per time
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