重组人白细胞介素23（IL-23）

重组人白细胞介素23（IL-23）

Recombinant Human IL-23 (C-6His) (CW54)

产品说明(Description)

Recombinant Human Interleukin-23 is produced by our Mammalian expression system and the targetgene encoding Ile23-Ser328&Arg20-Pro189 is expressed with a 6His tag at the C-terminus.

Accession #: P29460&Q9NPF7

Known as: SGRF;IL-23p19;CLMF p40;IL-12 subunit p40;NKSF2

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

质量控制(Quality Control)

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity: Measured by its ability to induce STAT reporter activity in 293F human embryonic kidney cells. The ED50 for this effect is 300-900 ng/ml.

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

背景(Background)

Interleukin 23 (IL-23) is a heterodimeric cytokine composed of two disulfide-linked subunits, a p19 subunit that is unique to IL-23, and a p40 subunit that is shared with IL-12. The p19 subunit has homology to the p35 subunit of IL-12, as well as to other single chain cytokines such as IL-6 and IL-11. The p40 subunit is homologous to the extracellular domains of the hematopoietic cytokine receptors. Although p19 is expressed by activated macrophages, dendritic cells, T cells, and endothelial cells, only activated macrophages and dendritic cells express p40 concurrently to produce IL-23. IL-23 has biological activities that are similar to, but distinct from IL-12. Both IL-12 and IL-23 induce proliferation and IFN-gamma production by human T cells. While IL-12 acts on both naive and memory human T cells, the effects of IL-23 is restricted to memory T cells. 白细胞介素23（IL-23）是由IL-23A（p19）和IL-12B（p40）两个亚基通过二硫键连接形成的异源二聚体细胞因子。p19亚基与IL-12的p35亚基以及其他单链细胞因子比如IL-6和IL-11均具有同源性，p40亚基与造血细胞因子受体的胞外结构域同源。人p19 cDNA编码具有189个氨基酸的前体蛋白，其具有19个氨基酸信号肽和170个氨基酸成熟蛋白。人和小鼠p19具有70％的氨基酸序列同源性，p19可以由活化的巨噬细胞、DC细胞、T细胞和内皮细胞表达产生，但是仅有活化的巨噬细胞和DC细胞能同时表达p40以产生具有生物学活性的IL-23
注意：FOR RESEARCH USE ONLY仅限科学研究用，不得用于临床！