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PIK3R1 Rabbit Polyclonal Antib

ody抗体,orb11271,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb11271
  • 英国
  • 2025年12月03日
  • FC, ICC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Mouse, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      PIK3R1 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      PIK3R1 Rabbit Polyclonal Antibody

    • 靶点

      PIK3R1

    • 浓度

      1mg/ml

    • 应用范围

      FC, ICC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb11271

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from human PI3 kinase p85 subunit alpha (501-600/724aa)

    • 规格

      50 ul

    产品描述:PIK3R1 Rabbit Polyclonal Antibody

    别名:P85A_HUMAN; Phosphatidylinositol 3-kinase regulatory subunit alpha; GRB1; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; PtdIns-3-kinase regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha(PI3-kinase subunit p85-alpha; PtdIns-3-kinase regulatory subunit p85-alpha); PI 3-kinase p85α; PI 3-kinase p85 α; PI 3-kinase p85-α; SH3_PI3K_p85alpha; PI3-kinase p85 subunit alpha; phosphoinositide-3-kinase regulatory subunit 1; p85; AGM7; IMD36; p85-ALPHA; PI 3 Kinase p85 alpha;

    免疫原:KLH conjugated synthetic peptide derived from human PI3 kinase p85 subunit alpha (501-600/724aa)

    预测反应性:Bovine, Canine, Equine, Gallus

    分子量:80 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:29560125

    UniProt ID:P27986

    Note:For research use only.

    产品细节图片1
    paraffin embedded (mouse skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (orb11271) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片2
    Fixative-fixed, paraffin embedded (rat skeletal muscle), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (PI 3 Kinase p85 alpha) Polyclonal Antibody, Unconjugated (orb11271) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Positive control: H9C2 (2% Fixative-fixed), Isotype Control Antibody, Antibody: Rabbit IgG, Dilution: 1 µg in 100 µl 1 X PBS containing 0.5% BSA, Secondary Antibody, Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:200 in 1 X PBS containing 0.5% BSA, Primary Antibody, orb10295, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.

    产品细节图片4
    Sample: Heart (mouse) Lysate at 40 ug, Primary: Anti-PI3K p85 (orb11271) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 80kD, Observed band size: 85 kD.

    产品细节图片5
    Tissue/Cell: HepG2 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PI3K p85) polyclonal Antibody, Unconjugated (orb11271) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片6
    Tissue/Cell: NIH/3T3 cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (PI 3 Kinase p85 alpha) polyclonal Antibody, Unconjugated (orb11271) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

    产品细节图片7
    Tissue/Cell: rat brain tissue, 4% Fixative-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-PI3K/PI3 kinase p85 alpha subunit Polyclonal Antibody, Unconjugated (orb11271) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

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    图标文献和实验
    该产品被引用文献
    Meng, Yingying et al. Oleic acid stimulates HC11 mammary epithelial cells proliferation and mammary gland development in peripubertal mice through activation of CD36-Ca2+ and PI3K/Akt signaling pathway Oncotarget, 9, 12982-12994 (2018)
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    文献支持
    PIK3R1 Rabbit Polyclonal Antibody抗体,orb11271,biorbyt
    ¥3120