Recombinant Human TNFR2/CD120b Protein(aa 1-268,196 Met/Arg,His Tag)
库存:
99
供应商:
北京义翘神州科技股份有限公司
规格:
100 µg/1 mg
规格:
100 µg
产品价格:
¥3870.0
规格:
1 mg
产品价格:
¥25190.0
重组人 TNFR2/CD120b 蛋白(aa 1-268,196 Met/Arg,His标签)(产品说明)
蛋白名称:Human TNFR2 / CD120b / TNFRSF1B Protein (aa 1-268, 196 Met/Arg, His Tag)
蛋白构建:A DNA sequence encoding the human TNFRSF1B (EAW71731.1) (Met1-Arg268, natural variant 196 Met/Arg) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 95 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit TNF-α mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-40 ng/mL in the presence of 1 ng/mL recombinant human TNF-α.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Leu 23
蛋白分子量:The recombinant human TNFRSF1B comprises 257 amino acids and has a predicted molecular mass of 27.7 kDa. The apparent molecular mass of the protein is approximately 43 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号:EAW71731.1
蛋白氨基酸序列:Met1-Arg268
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
1, Zhao, XL; et al. Novel human three-domain antibody fragments against sTNFα as well as tmTNFα with high affinity generated by the combination of ribosome display and E.coli expression system. Scand. J. Immunol.