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Caspase 8 Rabbit Polyclonal An

tibody抗体,orb10241,biorbyt
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  • ¥3120
  • biorbyt已认证
  • orb10241
  • 英国
  • 2025年12月09日
  • FC, IF, IHC-Fr, IHC-P, WB
  • Rabbit
  • Human, Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体名

      Caspase 8 Rabbit Polyclonal Antibody抗体

    • 抗体英文名

      Caspase 8 Rabbit Polyclonal Antibody

    • 靶点

      CASP8

    • 浓度

      1mg/ml

    • 应用范围

      FC, IF, IHC-Fr, IHC-P, WB

    • 宿主

      Rabbit

    • 适应物种

      Human, Rat

    • 保质期

      6-12个月

    • 抗原来源

      详询

    • 目录编号

      orb10241

    • 级别

      科研级

    • 库存

      88

    • 供应商

      biorbyt

    • 标记物

      Unconjugated

    • 克隆性

      Polyclonal

    • 保存条件

      参考产品说明书

    • 形态

      Liquid

    • 亚型

      IgG

    • 免疫原

      KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10 (411-482/482aa)

    • 规格

      50 ul

    产品描述:Caspase 8 Rabbit Polyclonal Antibody

    别名:Caspase-8 p10; Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5.

    免疫原:KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10 (411-482/482aa)

    预测反应性:Bovine, Canine, Equine, Mouse, Porcine

    分子量:12/55 kDa

    应用稀释比例:WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg /Test

    防腐剂:0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.

    纯化:Affinity purified by Protein A

    保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

    Pubmed:26332509

    UniProt ID:Q9JHX4

    Note:For research use only.

    产品细节图片1
    Blank control (blue line): U251 (blue). Primary Antibody (green line): Rabbit Anti-caspase-8 antibody (orb10241), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, Dilution: 1 µg/test. Protocol, The cells were fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2% BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

    产品细节图片2
    Fixative-fixed, paraffin embedded (rat liver), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (orb10241) at 1:500 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

    产品细节图片3
    Sample: Jurkat (Human) Cell Lysate at 30 ug, K562 (Human) Cell Lysate at 30 ug, U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-caspase-8 subunit p18 (orb10241) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55/18 kD, Observed band size: 57 kD.

    产品细节图片4
    Tissue/cell: Hela cell, 4% Fixative-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (Caspase 8) polyclonal Antibody, Unconjugated (orb10241) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

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    图标文献和实验
    该产品被引用文献
    Barton, Samantha K. et al. Differential short-term regional effects of early high dose erythropoietin on white matter in preterm lambs after mechanical ventilation J Physiol, 594, 1437-1449 (2016)
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    资料下载:

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    Caspase 8 Rabbit Polyclonal Antibody抗体,orb10241,biorbyt
    ¥3120