- ￥6500
- biorbyt
- orb18863
- 英国
- 2025年12月02日
- ELISA, WB
- Goat
- Human, Mouse, Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体名：
Smooth muscle alpha-actin Antibody抗体
- 抗体英文名：
Smooth muscle alpha-actin Antibody
- 靶点：
Smooth muscle alpha-actin
- 应用范围：
ELISA, WB
- 宿主：
Goat
- 适应物种：
Human, Mouse, Rat
- 保质期：
6-12个月
- 抗原来源：
详询
- 目录编号：
orb18863
- 级别：
科研级
- 库存：
88
- 供应商：
biorbyt
- 标记物：
Unconjugated
- 克隆性：
Polyclonal
- 规格：
100 ug
别名:Anti-a actin antibody, anti-AAT6 antibody, anti-ACTA_HUMAN antibody, anti-ACTA2 antibody, anti-Actin alpha 2 smooth muscle aorta antibody, anti-Actin aortic smooth muscle antibody, anti-Actin, aortic smooth muscle antibody, anti-ACTSA antibody, anti-ACTVS antibody, anti-Alpha 2 actin antibody, anti-Alpha actin 2 antibody, anti-Alpha cardiac actin antibody, anti-alpha sma antibody, anti-Alpha-actin-2 antibody
预测反应性:Canine
分子量:42
应用稀释比例：ELISA: 1:128000, WB: 0.3-1 μg/ml
应用注释：ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.IHC: Successful IHC-P data obtained from a previous batch. An anonymous customer found positive results in IHC-P on Pig heart tissue.WB: Approx 45kDa band observed in Human Duodenum lysates (calculated MW of 42.0kDa according to NP_001604.1). Recommended concentration: 0.1-0.3 μg/ml.Experiment Notes: Immunofluorescence: see publication McIntire RH et al., Placenta. 2008 Jul;29(7):631-8. Epub 2008 Jun 5. (PMID: 18538388).
防腐剂:Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
纯化:Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
序列:EEEDSTALVC
保存说明:Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Pubmed:28705908
Entrez:59
Note:For research use only.
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文献和实验平滑肌 smooth muscle，plain muscle
即无纹肌（ non-striated muscle）的通称。被视为较横纹肌原始的一种肌肉。平滑肌除作为无脊椎动物的躯体肌而有广泛分布外，在脊椎动物除心肌之外而大部分内脏肌也是由平滑肌组成的。虽有如斧足类的闭壳肌和足系牵引肌等是由平行走向长纤维状细胞（平滑肌纤维）所构成，但多数的平滑肌则是由长纺锤形（脊椎动物的内脏肌长不到 1毫米）的单核细胞构成。它不构成独立的器官，而只是成为构成体壁和内脏壁的因素（肌层）。其细胞实质仅由相当于横纹肌的向异性物质组成，整体表现同样的双折射。在有的平滑肌中可
LABELING MUSCLE ACTIN WITH IATR
,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer 1 overnight
Labeling Muscle Actin with 5-iodoacetamidofluorescein
) 5.50Ti tubes,small vial. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT (100 mM stock)to 5 mM. 3.Dialyze against 250 ml buffer
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