- ¥2700
- CST
- USA
- 9441S
- 2025年07月16日
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Acetylated-Lysine Antibody #9441
- 库存：
1
- 供应商：
诺和致远
- 保存条件：
-20度
- 规格：
100ul
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry), ChIP=Chromatin IP, E-P=Peptide ELISA (DELFIA)
Specificity / Sensitivity
Acetylated-Lysine Antibody detects proteins posttranslationally modified by acetylation on the epsilon-amine groups of lysine residues. The antibody recognizes acetylated lysine in a wide range of sequence contexts. It has been demonstrated to recognize acetylated histones, p53, CBP, PCAF and chemically acetylated BSA. The antibody has been shown to react with as little as 0.04 ng of chemically acetylated BSA while not recognizing up to 25 µg of nonacetylated BSA. (U.S. Patent No's.: 6,441,140; 6,982,318; 7,259,022; 7,344,714; U.S.S.N. 11,484,485; and all foreign equivalents.)Acetylated-Lysine Antibody识别转录后修饰的赖氨酸残基ε-氨基乙酰化。此抗体可识别不同序列背景中的乙酰化赖氨酸。研究显示它可以识别乙酰化的组蛋白、p53、CBP、PCAF和化学乙酰化的BSA。抗体可以与少到0.04ng的化学乙酰化的BSA反应，却不能与非乙酰化的多到25ug的BSA反应。（美国专利号：6,441,140、6,982,318、7,259,022、7,344,714；U.S.S.N. 11,484,485；及其对应的国外部分）
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic acetylated lysine-containing peptide. Antibodies are purified by protein A and peptide affinity chromatography.该多克隆抗体用人工合成包含乙酰化赖氨酸的肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。
Western Blotting
Western blot analysis of extracts from NIH/3T3 cells, untreated or sodium butyrate-treated (5 mM for 24 hours), showing an increase in histone acetylation using Acetylated-Lysine Antibody.对NIH/3T3细胞抽提液，未处理或5 mM丁酸钠处理24小时，使用Acetylated-Lysine Antibody进行Western blot分析，显示组蛋白乙酰化增加。
IP
Western blot analysis of immunoprecipitated p53 showing an increase in p53 acetylation using Acetylated-Lysine Antibody (upper) or p53 antibody (lower). p53 was immunoprecipitated from lysates from 293 cells, untreated or UV-treated, using p53 Antibody #9282.对免疫共沉淀的p53，使用Acetylated-Lysine Antibody（上图）或p53 antibody（下图）进行Western blot分析，显示p53乙酰化增加。从293细胞裂解后免疫共沉淀的p53，未处理或UV处理，使用p53 Antibody #9282。
IHC-P (paraffin)
Immunohistochemical staining of a paraffin-embedded human breast tumor section showing nuclear and cytoplasmic localization of proteins with acetylated lysine residues using Acetylated-Lysine Antibody.对石蜡包埋的人乳腺肿瘤切片使用Acetylated-Lysine Antibody进行免疫组化分析，显示细胞核和细胞质定位。
Western Blotting
Western blot analysis of extracts from COS cells, untreated or TSA-treated, grown in 10% FBS (lanes 1 and 2) or serum starved for 18 hours (lanes 3 and 4), using Acetylated-Lysine Antibody (upper) or p44/42 MAP Kinase Antibody #9102 (lower).对COS细胞，未处理或TSA处理，在10% FBS（列1和列2）生长或血清饥饿处理18小时（列3和列4），使用Acetylated-Lysine Antibody（上图）或p44/42 MAP Kinase Antibody #9102（下图）进行Western blot分析。
Western Blotting
Specificity and sensitivity of Acetylated-Lysine Antibody assayed on acetylated BSA (4; 1; 0.2; 0.04 or 0.008 ng in lanes 1-5) or nonacetylated BSA (25,000; 5,000; 1,000 or 200 ng in lanes 6-9).Acetylated-Lysine Antibody的特异性或敏感性用乙酰化BSA（4、1、0.2、0.04或0.008 ng 列1-5）和非乙酰化BSA（25,000、5,000、1,000或200 ng 列6-9）进行检测。
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded NIH/3T3 untreated (left) or TSA-treated (right) using Acetylated-Lysine Antibody.对NIH/3T3细胞，未处理（左）或TSA处理（右），使用Acetylated-Lysine Antibody进行免疫组化分析。
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Acetylated-Lysine Antibody.对石蜡包埋的人结肠癌使用Acetylated-Lysine Antibody抗体进行免疫组化分析。
IF-IC
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or SAHA-treated (right), labeled with Acetylated-Lysine Antibody (green). Actin filaments have been labeled with Alexa Fluor R 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).对NIH/3T3细胞，未处理（左）或SAHA处理（右），使用Acetylated-Lysine Antibody（绿）进行共聚焦免疫荧光分析。肌动蛋白用Alexa Fluor® 555 phalloidin（红色）标记。蓝色伪彩=DRAQ5®#4084(荧光DNA染料)。
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 20 μl of Acetylated-Lysine Antibody or 2 μl of Normal Rabbit IgG #2729, using SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002. The enriched DNA was quantified by real-time PCR, using SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human MYT-1 Exon 1 Primers #4493. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.使用SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002试剂盒，将4 x 10^6 HeLa细胞的染色质，与20μl Acetylated-Lysine (Ac-K2-100) Rabbit mAb #9814或者2 μl of Normal Rabbit IgG #2729进行染色质免疫沉淀。使用人SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490和SimpleChIP® Human MYT-1 Exon 1 Primers #4493进行real-time PCR用来定量富集的DNA。每个样本中免疫沉淀的DNA代表了输入的染色质的量，相当于1。
Background
Acetylation of lysine, like phosphorylation of serine, threonine or tyrosine, is an important reversible modification controlling protein activity. The conserved amino-terminal domains of the four core histones (H2A, H2B, H3, and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (1). Signaling resulting in acetylation/deacetylation of histones, transcription factors, and other proteins affects a diverse array of cellular processes including chromatin structure and gene activity, cell growth, differentiation, and apoptosis (2-6). Recent proteomic surveys suggest that acetylation of lysine residues may be a widespread and important form of posttranslational protein modification that affects thousands of proteins involved in control of cell cycle and metabolism, longevity, actin polymerization, and nuclear transport (7,8). The regulation of protein acetylation status is impaired in cancer and polyglutamine diseases (9), and HDACs have become promising targets for anti-cancer drugs currently in development (10).赖氨酸的乙酰化如同丝氨酸、苏氨酸和酪氨酸的磷酸换一样，是一种控制蛋白活性的重要可逆手段。四个核心组蛋白（H2A, H2B, H3, H4）的保守氨基末端区域均包含赖氨酸，可以被组蛋白乙酰转移酶（HATs）乙酰化和被组蛋白脱乙酰酶（HDACs）去乙酰化（1）。组蛋白、转录因子和其它影响细胞内染色体结构、基因活性、细胞生长、分化和凋亡的蛋白都经乙酰化/去乙酰化的信号影响（2-6）。最近的蛋白组学研究显示赖氨酸残基的乙酰化是蛋白翻译后修饰的广泛而重要的手段，可以影响控制细胞周期、代谢、生存、肌动蛋白聚合和核转运相关的数千种蛋白。蛋白质乙酰化调节障碍在癌症和多聚谷氨酰胺疾病中发现（9），HDACs也成为目前正在开发的有希望的抗癌药物（10）。
Application References
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:IP
- Applications:IP
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:Western Blotting
- Applications:
- Applications:Western Blotting
- Applications:
- Applications:IP
- Applications:
- Applications:
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验-Lysine Antibody，能够特异识别Lysine的乙酰化，这类的抗体应该可以用来对细胞进行整体乙酰化水平的检测。cell signaling有在卖这类抗体，里面有提到可以做WB, IHC,IC等等，这些抗体说明后面有附上一些图和相关文献，你可以参考一下。 其中两个抗体： http://www.cellsignal.com/products/9441.html http://www.cellsignal.com/products/9681.html
Identifying Acetylated Proteins in Mitosis
to search for acetylated proteins in mitosis (2). First, we synchronized cells in mitosis and used a polyclonal anti-acetyl-Lysine antiserum to immunoprecipitate acetylated proteins, followed by their identification by LC-ESI-MS/MS. We then confirmed
【求助】谁有兔抗Paxillin和鼠抗phorsphotyrosine
kelvin12 请问各位同行谁有多余的兔抗Paxillin和鼠抗phorsphotyrosine，能否相赠？谢谢！ iammonkey kelvin12战友：你知道paxillin磷酸化的阻断剂是什么吗？正做相关东西，刚接触不太明白！ 磷酸化抗体 我公司有现货，具体如下： IBN4001 e-Acetylated Lysine Antibody IBN4006 e
