Monica Zhou ,(哈佛大学干细胞研究所)
We choose StemBeads FGF2 in order to use in combination with cell surface marker antibody for live cell immunostaining, and we have been very pleased with the product:
Stembeads FGF2 are stable with good quality, the beads gradually release FGF2 into the cell culture medium, which allow us to finish the assays in a 2-3 days timeframe without medium change.
Jack Sandoe ,(哈佛大学干细胞研究所Kevin Eggan实验室)
The StemBeads FGF2 worked really well in my hands. They allowed me to change the media every 3 or 4 days without any problems with differentiation. After a week and half of using StemBeads FGF2 I had beautiful plates of undifferentiated stem cells while using half the amount of media. This allowed me to spend more time doing important experiments and spend less money on expensive stem cell media.
Maureen Sherry Lynes (哈佛大学干细胞研究所Lee Rubin实验室)
In my hands, StemBeads work very well, even when cells are cultured on matrigel in using TESR. I have been feeding my cells very 3 days, and the look at least as good, if not better than cells fed every day without StemBeads. This allows me to take care of more cell lines at once, and to spend more time doing things besides routine tissue culture.
Gabsang Lee , (约翰霍普斯金大学医学院)
I am an Assistant Professor in Johns Hopkins University, School of Medicine. I have extensive experience of culturing human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) more than 7 years now. Luckily my lab had a chance to test FGF2 Beads for maintaining hESCs and hiPSCs. FGF2- beads showed successful results. Whenever I met someone who wants to start any research with hESCs and hiPSCs, they are worrying about tissue culture. Indeed these cells need very special care. One of the issues is that these cells should be fed everyday, which compromises several things, including work hours during weekend and holiday season, wasting expensive culture media and increasing chances of contamination. But the FGF2-beads can solve these issues and should be very attractive choice for the researchers who are working on these particular cell types.