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纽约
每天更换培养基?周末假日还要更换培养基?
培养基用的太快?太浪费?频繁换液,造成污染?
让StemBeads FGF2来帮您解决这些困扰!!!
StemBeads FGF2,这是来自纽约干细胞中心的科学家研发的产品。
原理:StemBeads FGF2 使FGF2稳定释放,给干细胞恒定的信号。
优势:
无需每天更换培养基,节省时间
大大节省干细胞培养基费用,节省费用
高质量培养干细胞,干细胞不会发生分化
持续释放FCF2因子,无需改变培养条件
使用方法:1 ml培养基中只需加入7.5uls的StemBeads
美国著名干细胞研究中心的客户反馈:
Monica Zhou ,(哈佛大学干细胞研究所)
We choose StemBeads FGF2 in order to use in combination with cell surface marker antibody for live cell immunostaining, and we have been very pleased with the product:
Stembeads FGF2 are stable with good quality, the beads gradually release FGF2 into the cell culture medium, which allow us to finish the assays in a 2-3 days timeframe without medium change.
Jack Sandoe ,(哈佛大学干细胞研究所Kevin Eggan实验室)
The StemBeads FGF2 worked really well in my hands. They allowed me to change the media every 3 or 4 days without any problems with differentiation. After a week and half of using StemBeads FGF2 I had beautiful plates of undifferentiated stem cells while using half the amount of media. This allowed me to spend more time doing important experiments and spend less money on expensive stem cell media.
Maureen Sherry Lynes (哈佛大学干细胞研究所Lee Rubin实验室)
In my hands, StemBeads work very well, even when cells are cultured on matrigel in using TESR. I have been feeding my cells very 3 days, and the look at least as good, if not better than cells fed every day without StemBeads. This allows me to take care of more cell lines at once, and to spend more time doing things besides routine tissue culture.
Gabsang Lee , (约翰霍普斯金大学医学院)
I am an Assistant Professor in Johns Hopkins University, School of Medicine. I have extensive experience of culturing human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) more than 7 years now. Luckily my lab had a chance to test FGF2 Beads for maintaining hESCs and hiPSCs. FGF2- beads showed successful results. Whenever I met someone who wants to start any research with hESCs and hiPSCs, they are worrying about tissue culture. Indeed these cells need very special care. One of the issues is that these cells should be fed everyday, which compromises several things, including work hours during weekend and holiday season, wasting expensive culture media and increasing chances of contamination. But the FGF2-beads can solve these issues and should be very attractive choice for the researchers who are working on these particular cell types.
订购信息
货号 名称 规格
SKU: SB501 SteamBeads FGF2 Trial Size 1ml
SKU: SB500 StemBeads FGF2 3ml
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文献和实验浅析染色质免疫沉淀(ChIP)技术在 DNA 与蛋白质相互作用研究中的重要性
[4] 。由于在信号转导和表观遗传研究中的突出作用,ChIP 在肿瘤 [5-7] 、神经科学 [8-10] 、植物发育 [11-13] 等领域中应用非常广泛,同时有关细胞凋亡 [14] 、雌激素信号转导 [15] 、胰岛素抵抗 [16] 、组织发育 [1] 的文献中也用到ChIP。 目前,最常见的有以下两种 ChIP 实验技术: nChIP:用来研究 DNA 及高结合力蛋白,采用微球菌核酸酶(micrococcal nuclease)消化染色质,然后进行片段富集及后续分析,适用于组蛋白及其异构体,例如 [17
Microarray Approaches for Analysis of Cell Cycle Regulatory Genes
pRB SBF (Swi4, Swi6) E2F-1, E2F-2 MBF (Mbp1, Swi6)
浅析染色质免疫沉淀(ChIP)技术在DNA与蛋白质相互作用研究中的重要性
nChIP:用来研究DNA及高结合力蛋白,采用微球菌核酸酶(micrococcal nuclease)消化染色质,然后进行片段富集及后续分析,适用于组蛋白及其异构体,例如 [17-19] ; 2. xChIP:用来研究DNA及低结合力蛋白,采用甲醛或紫外线进行DNA和蛋白交联,超声波片段化染色质,然后进行片段富集及后续分析,适用于多数非组蛋白的蛋白,例如 [9, 15, 20] 。 X-ChIP试验的一般过程
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