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文献和实验Sample Protopcol for Two-Step Carbodiimide Coupling of Protein to MagPlex™ Magnetic Carboxylated Microspheres Microspheres should be protected from prolonged exposure to light throughout this procedure. 1.Resuspend
, we outline in detail an innovative lentiviral-based functional proteomic approach that can be used to rapidly characterize protein complexes from a broad range of mammalian cell lines. This method integrates the following key features: (1) lentiviral elements
A Novel Method for Determining the Kinetics of G Protein-Coupled Receptor Plasma Membrane Expression
of receptors can be monitored using radioligand binding or microscopy or both techniques. Using this new approach it should be possible to determine the rate of plasma membrane delivery of a broad range of G protein-coupled receptors
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