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- EN0581
- 2025年07月16日
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文献和实验Generating Nested Deletions with Exonuclease III
Exonuclease III (Exo III) will digest double-stranded DNA in a 3′ to 5′ direction if the DNA is blunt ended or possesses a 5′ overhang. It will not digest if there is a 3′ overhang of three or more bases, or if the 3′ end has had thiophosphate
The protocol for LIC by Exonuclease III
The protocol for LIC by Exonuclease III 梁耀极 1. Design the primers with 15-bp overlap; 2. Digest the vector by proper restriction enzyme; For getting high quality vectors, there are some good advices as follows: 1) The restriction
point. Add 4U Exonuclease III/ml reaction mix and incubate @ 37℃ (‰400 digested/min). 4. Remove 5ml aliquots into the S1 nuclease tubes (on ice) at various times. When all time points have been collected, incubate the tubes 30' @ RT. 5. Add 2ml STOP
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