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- Invitrogen
- 美国
- 182410
- 2025年07月16日
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文献和实验Guide to Techniques in Creating Transgenic Mouse Models Using SV40 T Antigen
The experimental introduction of foreign DNA into the germ line of a mouse is a powerful tool used to identify genetic elements required for appropriate tissue expression, to determine the developmental specificity of gene expression
of the House Mouse, Mus Musculus Genome number of chromosomes 40 diploid DNA content about 6 pg per cell 3X109 bp per haploid genome recombination units 1600 centimorgans (2000 Kb/cM) approximate number of genes§ 0.5 - 1.0 X 105 percent of genome
Lentiviral Gene Transduction of Mouse and Human Stem Cells
transfection and for measuring viral titers. Methods to concentrate viral supernatants to achieve a higher titer are also described. The protocols given here have been used successfully to transduce engrafting mouse and human HSCs as well as progenitor cells
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