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文献和实验Dendritic cells are the key initiators and organizers of the immune response. They are specialized antigen-presenting cells whose immunogenicity leads to the induction of antigen-specific immune responses. They are therefore ideal vehicles
Raf-1 Protein Kinase Activity in T Cells from Aged Mice
Most of our models of signal transduction through the T-cell receptor (TCR) involve components and pathways first described in T-cell clones and T-cell lymphomas such as the Jurkat cell line (1 ). These studies, while providing valuable
Proteomic Detection of Oxidized and Reduced Thiol Proteins in Cultured Cells
, the reduced thiols are first blocked with N -ethyl-maleimide, then the oxidized thiols reduced with dithiothreitol and labelled with 5-iodoacetamidofluorescein. The method is illustrated by treating Jurkat T-lymphoma cells with hydrogen peroxide and monitoring
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