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- Invitrogen
- 美国
- F14201
- 2025年12月27日
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文献和实验Apoptosis detection methodology is an ever evolving science. The caspase family of cysteine proteases plays a central role in this environmentally conserved mechanism of regulated cell death. New methods that allow for the improved detection
Measurement of Ca2+ Fluxes in Permeabilized Cells Using 45 Ca2+ and Fluo-3
Many cell-surface receptors via G-proteins activate phosphoinositidase C, which catalyzes the hydrolysis of phosphatidylinositol 4,5-bis-phosphate to produce the second messengers, myo-inositol 1,4,5-trisphosphate [Ins(1,4,5)P3 or IP
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, which cleaved the ester bond and left the calcium-sensitive free acid trapped within the cell (5 ). A number of improved calcium indicators have since been developed (e.g., fura-2, indo-1, and fluo-3), but the basic principles of dye loading and continuous
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