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- 2025年12月27日
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文献和实验Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, which cleaved the ester bond and left the calcium-sensitive free acid trapped within the cell (5 ). A number of improved calcium indicators have since been developed (e.g., fura-2, indo-1, and fluo-3), but the basic principles of dye loading and continuous
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Use of Fura-2 in whole cell suspensions to measure changes in intracellular Ca2+ is probably one of the simplest, yet most widely used protocols described in this volume. Whole cell suspensions are loaded with Fura-2 and then placed
Apoptosis detection methodology is an ever evolving science. The caspase family of cysteine proteases plays a central role in this environmentally conserved mechanism of regulated cell death. New methods that allow for the improved detection
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