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文献和实验Determining Relative Transduction of Adherent Cells
poorly in stem cells, lymphocytes, and primary neurons. For these cell lines observed GFP signal significantly underestimates the transduced population. Thus optimal transduction conditions can still be determined, but transfection efficiency
BacMam-Mediated Gene Delivery into Multipotent Mesenchymal Stromal Cells
Baculoviruses have been used over the last several decades for high-level protein production in insect cells. Recently, modified baculovirus containing a mammalian promoter, known as BacMam virus, has been shown to give high transduction
Toll-Like Receptor Interactions Imaged by FRET Microscopy and GFP Fragment Reconstitution
Protein–protein interactions regulate biological networks. The most proximal events that initiate signal transduction frequently are receptor dimerization or conformational changes in receptor complexes. Toll-like receptors (TLRs
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