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96 Tips / 盒, 16 盒 / 箱
货号:TT-1000-C-HTR-S
包装规格:96 Tips / 盒, 16 盒 / 箱
品牌:Axygen
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. Centrifuge at maximum speed ($13,000 x g) for 3 minutes at room temperature Carefully discard supernatant. 24. Air dry the DNA pellet by inverting the tube on a absorbent paper for 5-10 minutes. 25. Add 100ul EB Buffer to the tube and incubate
, and ethanol to remove potential fluorescent contaminants prior to taping. Denaturing 6% polyacrylamide gels are poured into 0.3 mm X 89 cm X 52 cm taped plates and fitted with 36 well forming combs. After polymerization, the tape and the comb are removed
the LCM procedure.TIP: The number of cells needed to successfully perform the assay varies depending on the quality and processing conditions of the tissue samples. One thousand cells is recommended as a good starting point.Suspend approximately 1000
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