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999
- 供应商：
北京泽平
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货号：T_70110-700-100
中文名称：
英文名称：TECAN STYLE 1000UL STERILE TIP
货期：热销品现货，其他请咨询
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文献和实验700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum
700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs is optimum
Large-scale yeast transformation
. We omitted this for months. It didn't seem to affect transformation efficiency at all, but maybe it affects non-homologous integration? Large Scale Transformation Dispense yeast competent cells into deepwells with DNA (100 ul/well).
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