5 ML pipette tips, Racked.

Practical Tips for Handling RNA

volumes (10 ml or greater), a short incubation on ice (5 minutes) can help compact the residue at the interface. Ethanol Wash Ethanol washes are performed after salt/EtOH precipitations to remove any residual salt from the nucleic acid pellet

Yale RNA prep

Yale RNA prep (Rebecca D. Burdine, Michael J. Stern [modified by S.M. Hettenbach]) Add 8 ml of TRIZOL to 2 ml packed worms in 15 ml centrifuge tube. Vortex and invert tube to solubilize and lyse worms for at least 10 min.

PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

of No. 30 G 1/2 needle is cut off and then rounded using sharpening stone);       1 ml syringe;       Dissecting instruments (fine-pointed scissors, fine forceps);       No. 5 forceps (Dumont);       Mouth pipette (aspirator mouth piece, latex tubing, blue