10 ML pipette tips, racked

Practical Tips for Handling RNA

volumes (10 ml or greater), a short incubation on ice (5 minutes) can help compact the residue at the interface. Ethanol Wash Ethanol washes are performed after salt/EtOH precipitations to remove any residual salt from the nucleic acid pellet

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

8.0 by gently pipetting up and down with a 10 ml pipette do not vortex. Cells should be resuspended completely. The cells suspend more efficiently with 10 mM EDTA alone (rather than with GET 50 mM glucose 25 mM Tris-HCl, pH 8.0 and 10 mM of EDTA, pH

热激转化(DH10B)

2 and pipette gently up and down. Total volume will be approximately 130 μl.3) Add 50-60 μl of resuspend cells to each ligation. Tap gently to mix.4) Leave on ice for 15 min.5) Heat shock for 90 sec at 42 ℃.6) Add 1 ml pre-warmed or RT LB.7) Put tubes in 37 ℃