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文献和实验suspension to 20% transmittance using Biomerieux （Vitek）colorimeter. 2.5 Transfer 200 μl suspension to labeled 1.5 ml sterile microfuge tubes using p1000 pipette to reduce DNA shearing. 2.6 Add 10μl of Proteinase K （20mg/ml stock） to each tube and mix
秀丽线虫RNA提取--C. elegans RNA prep
, and to help you to see the RNA pellet in the bottom. 12. Using an RNase free 200 µl pipette tip, VERY CAREFULLY remove the remaining liquid. The clear gelatinous RNA may be floating or in several pieces at this point; sometimes it helps to tilt the tube
before and after the spin. It's OK to do the spin for a few more or less hours if necessary. 9. After the spin, carefully remove the tubes from the buckets into a rack, taking care not the disturb the gradient. The brownish proteins should still be above the felt tip
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