赛默飞世尔Thermo Fisher X960 200µL

Karyotyping of X. tropicalis

with Hoechst! 14. Tip the slide up and allow stain to run off the slide and onto the paper towel. 15. Mount by placing a drop of PBS/glycerol on slide, add large coverslip and seal edges with clear nail polish. 16. Examine slide for presence

ChIP protocol for X. laevis Lens1/FoxE3 enhancer

SDS lysis buffer/1x proteinase inhibitor cocktail (2.5 µl of 200 x stock from Upstate EZ ChIP kit). Roughly dissociate heads by pipetting, and transfer them into a 2 ml dounce homogenizer. Total volume is ~700 µl. �SDS lysis

Mapping Protein Distributions on Polytene Chromosomes by Immunostaining

. Shake rack thoroughly during the washing procedure. iv. Rinse slides in PBS. Add 100 µL of a solution containing 0.5 mg/mL DAB and 0.01% H2 O2 . v. To prevent overstaining, follow the appearance