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- Axygen
- SCT-150-L-C-S
- 2025年07月11日
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5 小袋 / 盒, 8 盒 / 箱
货号：SCT-150-L-C-S
包装规格：5 小袋 / 盒, 8 盒 / 箱
品牌：Axygen
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文献和实验Materials2 ml size Sarstedt screw cap tubesBIO-RAD Disposable Chromatography Column （BIO-RAD cat. #732-6008）Oligo dT celluloseRNase-free water5.0 M NaCl0.5 M EDTA1.0 M Tris, pH 7.410% SDS70% EtOHBefore starting protocol1. Measure out 75 mg of oligo
culture hood. Ensure that any alcohols and wrapping paper are kept clear of the bunsen burner. Pre-sterilize the hood before use, and use disposable sterile gloves. Loosen the cap of a tissue culture flask and the cap of a stock bottle of tissue culture
In vitro transcription reaction
. A- Set up the temperature in hybridization incubator. 62 oC B- Place the dry membrane (up to 4) in a hybridization screw cap tube. Pre-hybrid. Stock solution ml [final concentration] DECP DDW 0.8 Deionized formamide 5 50 % 20X SSPE 2.5 5X 50
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