Agarose LE, Low EEO, Molecular

PCR product purification-Molecular Biology

product on agarose gel 1% check you DNA band.If your DNA band is too low, pool 2 or 3 PCR and run on agarose gel for purification.Use the minimum agarose% to minimise lost of sample.I'm using Qiagen minielute and it give me great results in a range of 400

STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS

and to use PCR to determine that the cells contain your construct. PCR is necessary here because the Ti plasmid is single copy and you can barely see it on agarose gels. 1. Grow cells overnight in 5 ml LB or YEP with antibiotics. For pMONs in ABI - 50

Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans

Adjust the Z focus of the expanded beam by moving lens L3 of the Galilean telescope (fig.2). 3. Laser axotomy and time-lapse microscopy of axon regeneration    1)      Microwave 10% Agarose (RPI Molecular Biology Grade EEO 0.1 A20090