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文献和实验Cyanine Dye Purification Protocol
Sep-Pak purified material, keeping the dyes separate (see Lyophilization and Redilution section ). Resuspend with a small volume (200-500 ml) of 0.2 M TEAA. Centrifuge the sample through a 0.45 mm filter. Check the absorbance at 260
Amplification and Labelling with Cy dyes
thermalcycler for the following conditions: 30sec. 92℃ 30sec. 40℃ 30sec. 50℃ 1min. 72℃ repeat cycle 24 times 3.Second round of PCR amplification to incorporate Cy dyes Prepare the following: F-100X dNTPs: 10ml 100mM dATP 10ml 100mM dCTP 10ml 100mM
DiOlistics: Delivery of Fluorescent Dyes into Cells
DiOlistic labeling utilizes a particle-mediated delivery system to incorporate dye into cells. Because of its random nature, this technique generates sparse fluorescent labeling which is well suited for the study of neuronal dendritic
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