Dye Labelled Nucleid Acids

Single-Molecule Fluorescence of Nucleic Acids

Single-molecule fluorescence studies of nucleic acids are revolutionizing our understanding of fundamental cellular processes related to DNA and RNA processing mechanisms. Detailed molecular insights into DNA repair, replication

Taq-polymerase catalyzed cycle sequencing using fluorescent-labeled dye terminator reactions

in the reaction mix. We have recently observed that the nested fragment set distribution for the DNA cycle sequencing reactions using the fluorescent labelled terminators (8) is much less sensitive to DNA concentration than that obtained with the fluorescent

Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids

A novel method for DNA enzymatic cleavage assays using molecular beacons (MBs) as the substrate for nuclease is described. An MB is a hairpin-shaped DNA probe that is labeled with a fluorescent dye at one end and a quencher at the other end