多核苷酸激酶

Polynucleotide Kinase

from Escherichia coli E. D. pFDX

别名:
kinase, polynucleotide

一般描述

Polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5′-hydroxyl terminus of DNA or RNA. It also can catalyze the exchange of 5′-terminal phosphate groups. In addition, the enzyme has a 3′-phosphatase activity (only at low pH).

应用

Use this polynucleotide kinase to:

• Phosphorylate 5′ ends of DNA or RNA
• Label 5′-hydroxyl ends of DNA or RNA with [γ-³²P]-ATP (either by direct phosphorylation or by phosphate exchange)
• Remove 3′-phosphates from RNA or DNA without altering their 5′ ends (at low pH only)

特点和优势

Contents
Solution, containing 10U/μl of enzyme, in Storage buffer:
50mM Tris-HCl, 1mM dithiothreitol, 0.1mM EDTA, 0.1μM ATP, 50% glycerol (v/v), pH approx. 7.5 (+25°C).
Phosphorylation buffer 10x concentrated:
10x Incubation buffer for direct phosphorylation, supplied with the enzyme: 500mM Tris-HCl, 100mM MgCl₂, 0.1mM EDTA, 50mM dithioerythritol, 1mM spermidine, pH 8.2 (at +25°C).

质量

Absence of endonucleases, nicking activity, exonucleases, and ribonucleases tested according to the current Quality Control procedures. Function tested by DNA 5′-end labeling.

单位定义

One unit is the enzyme activity which catalyzes the incorporation of 1 nmol ³²P into acid-precipitable products within 30 minutes at +37 °C.

制备说明

Activator: Polymines (spermine, spermidine)

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