Polynucleotide kinase catalyzes the transfer of the terminal phosphate group of ATP to the 5′-hydroxyl terminus of DNA or RNA. It also can catalyze the exchange of 5′-terminal phosphate groups. In addition, the enzyme has a 3′-phosphatase activity (only at low pH).
应用
Use this polynucleotide kinase to:
Phosphorylate 5′ ends of DNA or RNA
Label 5′-hydroxyl ends of DNA or RNA with [γ-32P]-ATP (either by direct phosphorylation or by phosphate exchange)
Remove 3′-phosphates from RNA or DNA without altering their 5′ ends (at low pH only)
特点和优势
Contents Solution, containing 10U/μl of enzyme, in Storage buffer:
50mM Tris-HCl, 1mM dithiothreitol, 0.1mM EDTA, 0.1μM ATP, 50% glycerol (v/v), pH approx. 7.5 (+25°C).
Phosphorylation buffer 10x concentrated:
10x Incubation buffer for direct phosphorylation, supplied with the enzyme: 500mM Tris-HCl, 100mM MgCl2, 0.1mM EDTA, 50mM dithioerythritol, 1mM spermidine, pH 8.2 (at +25°C).
质量
Absence of endonucleases, nicking activity, exonucleases, and ribonucleases tested according to the current Quality Control procedures. Function tested by DNA 5′-end labeling.
单位定义
One unit is the enzyme activity which catalyzes the incorporation of 1 nmol 32P into acid-precipitable products within 30 minutes at +37 °C.
制备说明
Activator: Polymines (spermine, spermidine)
10633542001
Polynucleotide Kinase, 1000 U多核苷酸激酶
1,000 U
11209299001
Nylon Membranes, 20 sheets 尼龙膜
20 sheets (10 x 15 cm)
11585614910
DIG High Prime Lab./Det.Kit II
1 kit (12 labeling reactions and 24 detection reactions)