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文献和实验Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Monoclonal Antibody Production
Harvest more feeder cells for your next expansion. Put 0.5 mL f feeder cells in DMEM/20 FCS (note the removal of the HT) in a T25 flask (one for each positive clone) for later use When the cells are ready for expansion out of the 24-well plate
Production of Monoclonal Antibody by DNA Immunization with Electroporation
DNA immunization with in vivo electroporation is an efficient alternative protocol for the production of monoclonal antibodies (mAb). Generation of mAb by DNA immunization is a novel approach to circumvent the following technical hurdles
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