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文献和实验Sampling and Analysis of Phloem Sap
However, in many studies the important analysis of phloem sap is neglected, probably because the content of sieve tubes is not easy to access. This chapter will describe the current methods for sampling and analysis of phloem sap in order
up by the sampling that they are hard to resample should you need to do this.Option 2: Grow your colonies in microfuge tubes in 200 µl (or less?) LB broth with 50 µg/ml ampicillin at 37℃ in the shaking incubator for 1 or 2 days. E. coli clumps can be dispersed
reaction. This has the advantage of being quick and easy, but the colonies are frequently so messed up by the sampling that they are hard to resample should you need to do this. Option 2: Grow your colonies in microfuge tubes in 200 µl (or less?) LB
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